p irf3 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc p irf3
P Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p/pmc12989726-434-17-26
Average 86 stars, based on 1 article reviews

p irf3 - by Bioz Stars, 2026-10

86/100 stars

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Blocking Assay:

Article Title: Radiosensitizing Effect of PARP Inhibition on Chondrosarcoma and Chondrocyte Cells Is Dependent on Radiation LET
Article Snippet: The extracted samples were then separated by SDS-PAGE on a TGX 4–15% gradient polyacrylamide gel (Bio-Rad, Marnes-la-Coquette, France) and transferred to a polyvinylidene difluoride (PVDF) membrane. .. Membranes were blocked by Azure Fluorescent Blot blocking buffer (Azure Biosystems, Dublin, OH, USA) for 1 h. Membranes were incubated with anti-Poly-ADP-ribose (1:1000, MABC547 Merck, Damstadt, Germany), anti-Poly-ADP-Ribose-Protein (1:1000, #90959, Merck, Darmstadt, Germany) anti-p.ATM (S1981) (D25E5) (1:1000, #13050, Cell Signaling, Danvers, MA, USA), anti-p.BRCA1 (1:1000, S1524) (#9009, Cell Signaling, Danvers, MA, USA), anti-p. DNAPK (S2056) (1:1000, AB124918, Abcam, Cambridge, UK), or anti-beta-actin (1:5000, A1978, Merck, Darmstadt, Germany) diluted in TBS-T with 1% skim milk powder overnight at 4 °C. .. Then, the blots were washed 3 times with TBS-T and then incubated with goat-anti-Rabbit Azure Spectra 700 (1:10,000, Azure Biosystems, Dublin, OH, USA) or with goat-anti-Mouse Azure Spectra 800 (1:10,000, Azure Biosystems, Dublin, OH, USA) diluted in TBS-T with 1% skim milk powder for 1 h. Blots were washed 3 times with TBS-T before reading their infrared signals with Ultimate Western Blot Imager Azure 600 (Azure Biosystems, Dublin, OH, USA).

Article Title: Radiosensitizing Effect of PARP Inhibition on Chondrosarcoma and Chondrocyte Cells Is Dependent on Radiation LET
Article Snippet: The extracted samples were then separated by SDS-PAGE on a TGX 4–15% gradient polyacrylamide gel (Bio-Rad, Marnes-la-Coquette, France) and transferred to a polyvinylidene difluoride (PVDF) membrane. .. Membranes were blocked by Azure Fluorescent Blot blocking buffer (Azure Biosystems, Dublin, OH, USA) for 1 h. Membranes were incubated with anti-PolyADP-ribose (1:1000, MABC547 Merck, Damstadt, Germany), anti-Poly-ADP-Ribose-Protein (1:1000, #90959, Merck, Darmstadt, Germany) anti-p.ATM (S1981) (D25E5) (1:1000, #13050, Cell Signaling, Danvers, MA, USA), anti-p.BRCA1 (1:1000, S1524) (#9009, Cell Signaling, Danvers, MA, USA), anti-p. DNAPK (S2056) (1:1000, AB124918, Abcam, Cambridge, UK), or anti-beta-actin (1:5000, A1978, Merck, Darmstadt, Germany) diluted in TBS-T with 1% skim milk powder overnight at 4 ◦C. .. Then, the blots were washed 3 times with TBS-T and then incubated with goat-anti-Rabbit Azure Spectra 700 (1:10,000, Azure Biosystems, Dublin, OH, USA) or with goat-anti-Mouse Azure Spectra 800 (1:10,000, Azure Biosystems, Dublin, OH, USA) diluted in TBS-T with 1% skim milk powder for 1 h. Blots were washed 3 times with TBS-T before reading their infrared signals with Ultimate Western Blot Imager Azure 600 (Azure Biosystems, Dublin, OH, USA).

Incubation:

Article Title: Radiosensitizing Effect of PARP Inhibition on Chondrosarcoma and Chondrocyte Cells Is Dependent on Radiation LET
Article Snippet: The extracted samples were then separated by SDS-PAGE on a TGX 4–15% gradient polyacrylamide gel (Bio-Rad, Marnes-la-Coquette, France) and transferred to a polyvinylidene difluoride (PVDF) membrane. .. Membranes were blocked by Azure Fluorescent Blot blocking buffer (Azure Biosystems, Dublin, OH, USA) for 1 h. Membranes were incubated with anti-PolyADP-ribose (1:1000, MABC547 Merck, Damstadt, Germany), anti-Poly-ADP-Ribose-Protein (1:1000, #90959, Merck, Darmstadt, Germany) anti-p.ATM (S1981) (D25E5) (1:1000, #13050, Cell Signaling, Danvers, MA, USA), anti-p.BRCA1 (1:1000, S1524) (#9009, Cell Signaling, Danvers, MA, USA), anti-p. DNAPK (S2056) (1:1000, AB124918, Abcam, Cambridge, UK), or anti-beta-actin (1:5000, A1978, Merck, Darmstadt, Germany) diluted in TBS-T with 1% skim milk powder overnight at 4 ◦C. .. Then, the blots were washed 3 times with TBS-T and then incubated with goat-anti-Rabbit Azure Spectra 700 (1:10,000, Azure Biosystems, Dublin, OH, USA) or with goat-anti-Mouse Azure Spectra 800 (1:10,000, Azure Biosystems, Dublin, OH, USA) diluted in TBS-T with 1% skim milk powder for 1 h. Blots were washed 3 times with TBS-T before reading their infrared signals with Ultimate Western Blot Imager Azure 600 (Azure Biosystems, Dublin, OH, USA).

Article Title: Fatty Acid-binding Protein 4 Exacerbates Blood-brain Barrier Disruption Through the JNK/c-Jun/MMP12 Pathway After Traumatic Brain Injury.
Article Snippet: .. Supernatants were incubated for 2 h with 2 μg of anti-P–c-Jun antibody (91,952, Cell Signaling Technology) or isotype control IgG, followed by addition of Protein A/G Magnetic Beads (Thermo Fisher). ..

Binding Assay:

Article Title: Replication and extension of the subregion selectivity of glutamate-related changes within the nucleus accumbens associated with the incubation of cocaine-craving.
Article Snippet: .. The following rabbit polyclonal antibodies were used: anti-Homer2a/b (Cosmo Bio USA Inc., Carlsbad, CA, USA; 1:1000 dilution), anti-Homer1b/c (GeneTex Inc., Irvine, CA, USA; 1:1000 dilution), anti-mGlu5 (Millipore, Billerica, MA, USA; 1:1000 dilution), anti-GluN2a (Calbiochem, San Diego, CA, USA; 1:1000 dilution), anti-ERK1/2 (Santa Jo ur na l P re -p ro of Journal Pre-proof 9 Cruz Biotechnology, Santa Cruz, CA, USA; 1:2000 dilution), anti-PKCε (Santa Cruz Biotechnology; 1:1000 dilution), anti-p-(Ser729)PKCε (Santa Cruz Biotechnology; 1:500 dilution), anti-GluN2b (Calbiochem, San Diego, CA, USA; 1:1000 dilution), anti-PI3K antibody (Upstate Cell Signaling Solutions; Lake Placid, NY, USA; 1:1000 dilution), anti-p(Tyr)p85α PI3K binding motif to index PI3K activity (Zhang et al., 2006) (Cell Signaling Technology; Beverly, MA, USA; 1:500 dilution), anti-p(Ser473)Akt (Cell Signaling Technology; 1:250 dilution) and anti-Akt (Cell Signaling Technology; 1:1,000 dilution). .. The following primary mouse polyclonal antibodies were also used: anti-mGlu1α antibody (BD Transduction Laboratories; Franklin Lakes, NJ, USA; 1:500 dilution) and anti-p(Tyr204)ERK1/2 (Santa Cruz Biotechnology; 1:1000 dilution).

Article Title: KUS121, a novel VCP modulator, attenuates atherosclerosis development by reducing ER stress and inhibiting glycolysis through the maintenance of ATP levels in endothelial cells.
Article Snippet: .. The following primary antibodies were purchased and used: antiVCP (7F3, Cell signaling technology, #2649, 1:200), anti-BIP (Cell signaling technology, #3183, 1:200), anti-CHOP (L63F7, Cell signaling technology, #2895, 1:200), anti-p-JNK (Thr183/Tyr185) (81E11, Cell signaling technology, #4668, 1:200), anti-JNK (Cell signaling technology, #9252, 1:200), anti-Cleaved Caspase-3 (Cell signaling technology, #9664, 1:200), anti-p–NF–κB (Ser536) (93H1, Cell signaling technology, #3033, 1:200), anti–NF–κB (D14E12, Cell signaling technology, #8242, 1:200), anti-p-IRE1α (Ser724) (Novus, NB100-2323, 1:100), anti-IRE1α (14C10, Cell signaling technology, #3294, 1:200), anti-TBP (TATA Binding Protein) (N-12, Santa Cruz, sc-204, 1:500), anti-ACTB (AC-74, Sigma-Aldrich, A5316, 1:200). .. The secondary antibodies are as follows: anti-Rabbit IgG, HRP-Linked (GE Healthcare, NA934V, 1:500), anti-Mouse IgG, HRP-Linked (GE Healthcare, 931 V, 1:500).

Activity Assay:

Control:

Magnetic Beads:

Western Blot:

Article Title: Second-Generation Antipsychotics Induce Metabolic Disruption in Adipose Tissue-Derived Mesenchymal Stem Cells Through an aPKC-Dependent Pathway
Article Snippet: Lysotracker Deep Red was bought from Invitrogen (Invitrogen, Waltham, MA, USA), while Hoechst 33342 was bought from Sigma (Sigma-Aldrich, Saint Louis, MO, USA) and dissolved in H 2 O at a concentration of 5 mg/mL. .. For Western blot and immunofluorescence analyses, the primary antibodies were anti-Tubulin, anti-P-AKT S473, anti-P-AKT T308, anti-AKT, LAMP1 (Santa Cruz Biotechnology, Dallas, TX, USA) LC3B, (Sigma-Aldrich, Saint Louis, MO, USA), anti-P-INSRβ Y1146, anti-INSRβ, anti-P-ERK1/2, anti-ERK1/2, anti-RAB7, anti-P-(Ser)-PKC substrate (Cell Signaling Technology, Danvers, MA, USA), anti-p62, anti-PKCζ (Thermo Fisher Scientific, Waltham, MA, USA), anti-p-PKCζ T560 (Abcam, Cambridge, UK), and anti-CD63 (BD Biosciences, Franklin Lakes, NJ, USA). .. The secondary antibodies were goat anti-mouse IgG or donkey anti-rabbit IgG horseradish peroxidase (HRP); these were purchased from Perkin Elmer (PerkinElmer, Waltham, MA, USA).

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